Silabus Biotechnology Jurusan Pendidikan Biologi Semester 6 / VI

Faculty                                               :  Mathematics And Science

Study Program                                   :  Biology Education

Subject                                                :  Biotechnology

Credit                                                  :  Theory 2 unit of semester credit

Semester                                             :  VI

Prerequisite Subject                           : Biology Cell and Moleculer, Microbiology, Genetics

Lecturer                                            : Evy Yulianti, M.Sc., Paramita Cahyaningrum K., M.Sc., Lili Sugiyarto, M.Si.

I.         Subject Description

Biotechnology  study  the  technologies  that  involve  the  use  of  living  organisms  or products from living organisms in order to benefit humans usually for medical, agricultural and industrial application

II.       Standard of Competence

The main goal of this subject for students is to gain an understanding of the basic technique in biotechnology and the medical, agricultural and industrial application

III.       Lesson Plans

Meeting	Basic of Competence	Topics	Strategy	References	Character
1st	Describe what biotechnology is and the subject supporting biotechnology, describe the history of biotechnology	Introduction - Meaning of biotechnology - Subject supporting biotechnology - History of biotechnology	Lecturer, presentation and discussion		Appreciating diversity/appre ciation of diversity, Curious/curios ity, Passionate about learning/passi on for learning
2nd	Describe the PCR reaction mixture, condition and the use of PCR	Basic Technique in molecular Biology: PCR -   PCR reaction mixture -   PCR Conditions -   PCR-RFLP	Lecturer, presentation and discussion		Appreciating diversity/appre ciation of diversity, Curious/curios ity, Passionate about learning/passi on for learning

		-   Principles and Medical Applications of the Polymerase Chain Reaction
3rd	Describe how DNA being manipulated, explain kind of restriction enzyme and how they work, explain how to make recombinant DNA and the finction of recombinant DNA	Basic Technique in molecular Biology: DNA recombinant technology - The Manipulation of Nucleic Acids: Basic Tools and Techniques - Restriction Enzymes - Use of restriction enzymes - Recombinant DNA - Transformation of Bacteria - Constructing DNA libraries	Lecturer, presentation and discussion		Curious/curios ity, Passionate about learning/passi on for learning
4th	Explain biotechnology application in human gene therapy, IVF and DNA fingerprinting	Biotechnology application : in health and forensic -  Human Gene Therapy -  The technique of IVF -  DNA fingerprinting	Lecturer, presentation and discussion		Curious/curios ity, Passionate about learning/passi on for learning
5th	Explain biotechnology application in cloning	Biotechnology application : in livestock - Cloning	Lecturer, presentation and discussion		Curious/curios ity, Passionate about learning/passi on for learning
6th	Describe the history of plant biotechnology, methods used in plant biotechnology (tissue culture and transgenic)	Methods of Plant Biotechnology	Lecture and discussion	D and E (see list of references below)
7th	Describe the method of obtaining	Plant Transgenesis	Lecture and	D and E

	transgenic plants and the use of molecular markers		discussion	(see list of references below)
8th		Midterm I
9th	Describe the methods used for several examples of the application of plant biotechnology (vaccine for plants, genetic pesticides, herbicide resistance, enhanced nutrition, pharmacology, fuels)	Application of Plant Biotechnology	Lecture and discussion	D and E (see list of references below)
10th	Describe and analyze the negative and positive effects of biotechnology on the development of new plant varieties.	Health and environmental concerns	Lecture and discussion	D and E (see list of references below)
11th	Presentation
12 th		Microbial biotechnology (scope, technique) Microbial diversity Introduction of DNA into yeast cell	Lecture and discussion
13 th		Industrial application Fermentation process and fermenters Production of antibiotics Goals of antibiotic research	Lecture and discussion
14 th		Environtmental application The process of biodegradation Biodegradation of organic pollutants Bioremediation	Lecture and discussion
15 th		Human isulin gene Production of microbial enzyme Application of microbia enzyme	Lecture and discussion
16 th	Presentation

IV.      Reference

Compulsory reading  :

A. Agrawal,     S.  2008.  Techniques  in  Molecular  Biology.  International  Book

Distributing Co.

B. Popping  B,  Diaz-Amigo  C,  Hoenicke  K.  2010.  Molecular  Biological  And Immunological Techniques And Applications For Food Chemists.. John Wiley &Sons, Inc.

C. Walker, J. M., Rapley R. 2008 Molecular Biomethods Handbook. Humana Press, a part of Springer Science

D. Mantell,  S.H.,  J.A.Matthews,  and  R.A.McKee.  1985.  Principles  of  Plant

Biotechnology. Blackwell Scientific Publications. London. UK. 258p

E.  Renneberg,  R.  2006.  Biotechnology  for  Beginners.  (Ed.  Arnold.L.Demain).

Elsevier Inc. Muenchen.Germany.pp. 171-202.

F.  Syukur, M.,  S.Sujiprihati,  dan  R.Yunianti.2012.  Teknik  Pemuliaan  Tanaman.

Penebar Swadaya. Hal 173-184

G. Thieman,  W.J.,  and  M.A.Palladino.  2013.  Introduction  to  Biotechnology. Pearson Education, Inc. USA. Pp.158-174

V.        Evaluation

No	Component of evaluation	Score ( % )
1	Attendance	10
2	Participation	20
3	Assignment	20
4	Midterm	20
5	Final Examination	30
	Total	100